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Rabbit anti-BRCA2 Antibody, Affinity Purified

Catalogue number:
Size:
100 µl (1000 µg/ml) _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£436.00 Estimated delivery Wed 1 May
Shipping is calculated in checkout
Applications:

WB, IP

Antibody Host:

Rabbit

Target:

BRCA2

Species Reactivity:

Human

Antibody Type:

Polyclonal

Immunogen:

between 450 and 500

Alternative Names:

FAD1; FANCD; Fanconi anemia group D1 protein; mutant BRCA2; PNCA2; XRCC11; FANCD1; FAD; truncated breast cancer 2; BROVCA2; breast cancer type 2 susceptibility protein; BRCA1/BRCA2-containing complex, subunit 2; breast cancer 2, early onset; GLM3; breast

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Product Description:

Rabbit anti-BRCA2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)

Storage Temperature:

2 - 8°C

Detection of human and mouse BRCA2 by we
Detection of human and mouse BRCA2 by western blot. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg) and HeLa (15 and 50 µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRCA2 antibody A303-434A (lot A303-434A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human BRCA2 by western blot
Detection of human BRCA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRCA2 antibody A303-434A (lot A303-434A-2) used for IP at 6 µg per reaction. BRCA2 was also immunoprecipitated by a previous lot of this antibody (lot A303-434A-1) and rabbit anti-BRCA2 antibody A300-005A For blotting immunoprecipitated BRCA2, A303-434A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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