70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, epididymis secretory sperm binding protein, FPL, FPLD, FPLD2, HGPS, IDC, lamin, lamin A/C-like 1, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, mandibuloacral dysplasia type A, prelamin-A/C, PRO1, renal carcin
between 400 and 450
2 - 8°C
Detection of human Lamin-A by western blot and immunoprecipitation. Samples: Whole cell lysate (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Lamin-A antibody A303-432A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. Lamin-A was successfully immunoprecipitated by rabbit anti-Lamin-A/C antibody A303-430A, which recognizes an upstream epitope, and by rabbit anti-Lamin-A antibody A303-433A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 second (B).
Detection of human Lamin-A by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- Lamin-A (Cat. No. A303-432A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.