DAN, deadenylating nuclease, deadenylation nuclease, DKCB6, PFBMFT4, poly(A)-specific ribonuclease PARN, polyadenylate-specific ribonuclease
between 589 and 639
2 - 8°C
Detection of human PARN by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PARN antibody A303-562A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. PARN was also immunoprecipitated by rabbit anti-PARN antibody A303-561A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).