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Rabbit anti-RNF31 Antibody, Affinity Purified

Catalogue number:
A303-560A
Size:
100 µg
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£383.00 Estimated delivery Fri 28 Jan
Shipping is calculated in checkout

Rabbit anti-RNF31 Antibody, Affinity Purified - 100 µg

  • Applications: WB, IP
Product Type:

Antibodies

Alternative Names:

E3 ubiquitin-protein ligase RNF31, HOIL-1-interacting protein, HOIP, Paul, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, ZIBRA, zinc in-between-RING-finger ubiquitin-associated domain protein

Species Reactivity:

Human, Mouse

Immunogen:

between 1022 and 1072

Storage Temp:

2 - 8°C

Antibody Host:

Rabbit

Antibody Isotype:

Whole IgG

Antibody Type:

Polyclonal

UNSPSC Code:

12352203

Detection of human RNF31 by western blot

Detection of human RNF31 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF31 antibody A303-560A (lot A303-560A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of mouse RNF31 by western blot

Detection of mouse RNF31 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RNF31 antibody A303-560A (lot A303-560A-3) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human RNF31 by western blot

Detection of human RNF31 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RNF31 antibody A303-560A (lot A303-560A-3) used for IP at 6 µg per reaction. RNF31 was also immunoprecipitated by a previous lot of this antibody (lot A303-560A-3). For blotting immunoprecipitated RNF31, A303-560A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

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