dac homolog, DACH, Dach1, dachshund homolog 1
between 720 and 740
2 - 8°C
Detection of human DACH1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-DACH1 antibody A303-555A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. DACH1 was also immunoprecipitated by rabbit anti-DACH1 antibody A303-556A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).