factor inhibiting HIF1, factor inhibiting HIF-1, FIH1, FIH-1, hypoxia inducible factor 1 alpha subunit inhibitor, hypoxia-inducible factor 1-alpha inhibitor, hypoxia-inducible factor asparagine hydroxylase, peptide-aspartate beta-dioxygenase
between 300 and 349
2 - 8°C
Detection of human FIH-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-FIH-1 antibody A303-550A used for IP at 6 µg/mg lysate. For blotting immunoprecipitated FIH-1, rabbit anti-FIH-1 antibody BL12623, which recognizes and upstream epitope, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.