130 kDa cis-Golgi matrix protein, GM130, GM130 autoantigen, golgi autoantigen, golgin subfamily a, 2, Golgi matrix protein GM130, golgin subfamily A member 2, golgin-95, SY11 protein
between 952 and 1002
2 - 8°C
Detection of human GM130 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-GM130 antibody A303-402A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. GM130 was also immunoprecipitated by rabbit anti-GM130 antibody A303-401A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).