E(Sp1) homolog, enhancer of split groucho-like protein 1, ESG, ESG1, GRG1, transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila), transducin-like enhancer protein 1
between 175 and 225
2 - 8°C
Detection of human TLE1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TLE1 antibody A303-545A used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. TLE1 was also immunoprecipitated by rabbit anti-TLE1 antibody BL12605, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).