Rabbit anti-USF2 Antibody, Affinity Purified

Detection of human USF2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-USF2 antibody A303-541A used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. USF2 was also immunoprecipitated by rabbit anti-USF2 antibodies A303-540A and A303-542A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Detection of human USF2 by immunohistochemistry. Samples: FFPE serial sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti- USF2 (Cat. No. A303-540A Lot1, left image, A303-541A Lot1, middle image and Cat. No. A303-542A Lot1, right image) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse USF2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- USF2 (Cat. No. A303-541A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB