26S protease regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5, 26S proteasome regulatory subunit 6A, human immunodeficiency virus tat transactivator binding protein-1, proteasome (prosome, macropain) 26S subunit, ATPase, 3, Proteasome 26S sub
between 389 and 439
2 - 8°C
Detection of human and mouse TBP1 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HEK293T (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 µg), HeLa (H; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TBP1 antibody A303-538A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TBP1 was also immunoprecipitated by rabbit anti-TBP1 antibody A303-537A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).