CBTF, Double-stranded RNA-binding protein 76, double-stranded RNA-binding protein, 76 kD, DRBF, DRBP76, dsRNA binding protein NFAR-2/MPP4, interleukin enhancer binding factor 3, 90kD, interleukin enhancer binding factor 3, 90kDa, interleukin enhancer-bind
Between 652 and 702
2 - 8°C
Detection of human NF90 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa(15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-NF90 antibody A303-651A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NF90 was also immunoprecipitated by rabbit anti-NF90/NF110 antibodies A303-119A and A303-120A, which recognize upstream epitopes. NF90 was not immunoprecipitated by rabbit anti-NF110 antibody A303-122A. For blotting NF90 and NF110 (lower panel in B), A303-119A was used. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).