catechol O-methyltransferase, catechol-O-methyltransferase isoform, epididymis secretory sperm binding protein Li 98n, HEL-S-98n, testicular tissue protein Li 42
Between 221 and 271
2 - 8°C
Detection of human COMT by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-COMT antibody A303-631A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. COMT was also immunoprecipitated by rabbit anti-COMT antibody BL13028, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).