CXorf3, dJ506G2.1, hTREX120, mental retardation, X-linked 12, mental retardation, X-linked 35, MRX12, MRX35, THO complex subunit 2, THO2
Between 1400 and 1450
2 - 8°C
Detection of human THOC2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-THOC2 antibody A303-629A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. THOC2 was also immunoprecipitated by rabbit anti-THOC2 antibody A303-630A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).