WB, IP, IHC
surrounding threonine 286
MAPKK1; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1); PRKMK1; MKK1; MEK1; MEK 1; MAPKK 1; MAPK/ERK kinase 1; MAP kinase kinase 1; ERK activator kinase 1; CFC3; dual specificity mitogen-activated protein kinase kinase 1
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Goat anti-Phospho MEK1 (T286) Antibody, Affinity Purified - 100 µl (1000 µg/ml)
2 - 8°C
Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 µg HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified goat anti-phospho MEK1 (T286) A303-611A was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 (BL8445) was used for western blot (WB) at 1 µg/ml. Detection: Chemiluminescence with exposure time of 30 seconds.