Rabbit anti-Plakoglobin Antibody, Affinity Purified

Detection of human Plakoglobin by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Plakoglobin antibody A303-718A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Plakoglobin was also immunoprecipitated by rabbit anti-Plakoglobin antibody A303-717A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Detection of human Plakoglobin by immunohistochemistry. Sample: FFPE section of human prostate hyperplasia. Antibody: Affinity purified rabbit anti- Plakoglobin (Cat. No. A303-718A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB

Detection of human Plakoglobin by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- Plakoglobin (Cat. No. A303-718A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB