WB, IP, IHC
Between 350 and 400
TATA box binding protein (TBP)-associated factor 2D; transcription initiation factor TFIID subunit 5; transcription initiation factor TFIID 100 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD; TAFII-100; TAFII100
- Data sheet: View or download
Rabbit anti-TAF5 Antibody, Affinity Purified - 100 µl (1000 µg/ml)
2 - 8°C
Detection of human TAF5 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-TAF5 antibody A303-687A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. TAF5 was also immunoprecipitated by rabbit anti-TAF5 antibodies A303-685A and A303-686A, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Detection of human TAF5 by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- TAF5 (Cat. No. A303-685A Lot1, left image, A303-686A Lot1, middle image and Cat. No. A303-687A Lot1, right image) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).