WB, IP, IHC, F
Between 50 and 100
Bcl-2; B-cell CLL/lymphoma 2; apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50; PPP1R50
- Data sheet: View or download
Rabbit anti-Bcl-2 Antibody, Affinity Purified - 100 µl (1000 µg/ml)
2 - 8°C
Detection of human Bcl-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl-2 antibody A303-675A used for IP at 6 µg/mg lysate. Bcl-2 was also immunoprecipitated by rabbit anti-Bcl-2 antibody BL13035, which recognizes an upstream epitope. For blotting immunoprecipitated Bcl-2, A303-675A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.