Rabbit anti-TFEB Antibody, Affinity Purified
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Rabbit anti-TFEB Antibody, Affinity Purified - 100 µg
Antibodies
ALPHATFEB, BHLHE35, class E basic helix-loop-helix protein 35, T-cell transcription factor EB, TCFEB, transcription factor EB
Human
Between 325 and 375
2 - 8°C
Rabbit
Whole IgG
Polyclonal
12352203
Detection of human TFEB by western blot. Samples:Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFEB antibody A303-672A (lot A303-672A-3) used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 10 second.
Detection of human TFEB by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFEB antibody A303-672A (lot A303-672A-3) used for IP at 6 µg per reaction. TFEB was also immunoprecipitated by a previous lot of this antibody (lot A303-672A-2) and rabbit anti-TFEB antibody A303-673A For blotting immunoprecipitated TFEB, A303-672A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.