hU2AF(65), hU2AF65, splicing factor U2AF 65 kDa subunit, U2 (RNU2) small nuclear RNA auxiliary factor 2, U2 auxiliary factor 65 kDa subunit, U2 small nuclear ribonucleoprotein auxiliary factor (65kD), U2 snRNP auxiliary factor large subunit, U2AF65
Between 380 and 430
2 - 8°C
Detection of human U2AF65 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-U2AF65 antibody A303-666A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. U2AF65 was also immunoprecipitated by rabbit anti-U2AF65 antibodies BL13251 and A303-667A, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).