CTHBP, cytosolic thyroid hormone-binding protein, epididymis secretory protein Li 30, HEL-S-30, OIP3, OIP-3, OPA-interacting protein 3, p58, PK, muscle type, PK3, PKM2, pyruvate kinase 2/3, pyruvate kinase isozymes M1/M2, pyruvate kinase muscle isozyme, p
Between 481 and 531
2 - 8°C
Detection of human and mouse PKM2 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PKM2 antibody A303-660A used for WB at 0.1 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. PKM2 was also immunoprecipitated by rabbit anti-PKM2 antibody A303-659A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human PKM2 by immunohistochemistry. Samples: FFPE sections of human osteosarcoma. Antibody: Affinity purified rabbit anti- PKM2 Cat. No. A303-659A Lot1 used at a dilution of 1:1,000 (1µg/ml) (left) and Cat. No. A303-660A Lot1 used at a dilution of 1:5,000 (0.2µg/ml) (right). Detection: DAB