Antibody was affinity purified using an epitope specific to PCNA immobilized on solid support. The epitope recognized by A300-277B maps to a region between residue 225 and the C-terminus (residue 261) of human proliferating cell nuclear antigen using the numbering given in SwissProt entry P12004 (GeneID 5111). Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
ATLD2, cyclin, DNA polymerase delta auxiliary protein, PCNA, proliferating cell nuclear antigen
between 225 and C-term
2 - 8°C
Detection of human PCNA by western blot. Samples: Whole cell lysate from HeLa (50, 15, and 5 µg). Antibody: Affinity purified, biotin-conjugated, rabbit anti-Actin antibody (Cat. No. A300-277B) used at 1.0 µg/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.