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RedDot™2, 200X in DMSO

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25 ul _$$_
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£113.00 Estimated delivery Mon 8 Apr
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RedDot™2 is a far-red cell membrane-impermeable nuclear dye similar to Draq7™, with excitation/emission at 665/695 nm for detection in the Cy®5 channel. The dye is ideal for specifically staining the nuclei of dead cells with minimal cytoplasmic RNA staining. RedDot™2 provides excellent nuclear counterstaining in fixed and permeabilized cells and tissue sections without the need for RNase treatment to remove cytoplasmic RNA. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™2 also has been used for cell number normalization for near-infrared In Cell Western™. RedDot™2 can be efficiently excited by wavelengths from 488 to 647 nm, and therefore can be used with the 488 nm flow cytometry laser line.  Please note: far-red dyes like RedDot™2 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.  Please also see RedDot™1, a spectrally similar dye designed for specific nuclear staining of live cells.  In Cell Western is a trademark of LI-COR® Biosciences. Cy dye is a registered trademark of GE Healthcare. Draq5 is a trademark of BioStatus Ltd.

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Storage Temperature:

R

HeLa cells stained with CF405 phalloidin
HeLa cells stained with CF405 phalloidin (actin filaments, blue) and RedDot2 (nuclei, magenta).
MCF-7 cells stained with mouse anti-tubu
MCF-7 cells stained with mouse anti-tubulin and CF405M goat anti-mouse (microtubules, cyan). Nuclei are stained with RedDot2 (red).
MCF-7 cells stained with mouse anti-tubu
MCF-7 cells stained with mouse anti-tubulin and CF405M goat anti-mouse (microtubules, cyan). Nuclei are stained with RedDot2 (magenta).
HeLa cells stained with  rabbit anti-COX
HeLa cells stained with rabbit anti-COX IV and CF488A goat anti-rabbit (mitochondria, green), and mouse anti-Golgin 97 and CF555 goat anti-mouse (Golgi, red). Actin filaments are stained with CF405 phalloidin (blue) and nuclei are stained with RedDot2 (magenta).
HeLa cells stained with  rabbit anti-COX
HeLa cells stained with rabbit anti-COX IV and CF488A goat anti-rabbit (mitochondria, green), and mouse anti-Golgin 97 and CF555 goat anti-mouse (Golgi, red). Actin filaments are stained with CF405 phalloidin (blue) and nuclei are stained with RedDot2 (magenta).
Frozen section of rat optic nerve staine
Frozen section of rat optic nerve stained with mouse anti-neurofilament H and CF568 goat anti-mouse (min x rat) (neuronal processes, red), rabbit anti-GFAP and CF488A goat anti-rabbit, highly cross-adsorbed (glial cells, green). Nuclei are stained with RedDot2 (cyan). Mounted in Everbrite Mounting Medium.
BT40061-T: RedDot™2, 200X in DMSO
BT40061-T: RedDot™2, 200X in DMSO
BT40061-T: RedDot™2, 200X in DMSO
BT40061-T: RedDot™2, 200X in DMSO
BT40061-T: RedDot™2, 200X in DMSO
BT40061-T: RedDot™2, 200X in DMSO
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