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CF®790 Succinimidyl Ester

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Size:
250 nmol _$$_
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£228.00 Estimated delivery Mon 6 May
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CF® Dye Succinimidyl Esters (SE, also known as NHS esters) are amine-reactive fluorescent dyes. SE dyes are commonly used to label antibodies and other proteins on free amine groups, such as lysine residues. CF® dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Near-infrared fluorescent CF®790 dye has excitation/emission at 784/806 nm.

Storage Temperature:

F

Two dilutions of HeLa cell lysate (lanes
Two dilutions of HeLa cell lysate (lanes 2, 5: 1X lysate, lanes 3, 6: 1:5 dilution of lysate) were probed with mouse anti-tubulin antibody followed by goat anti-mouse conjugated to AlexaFluor 790 (AF790, lanes 1-3) or CF790 (lanes 4-6). Lanes 1 and 4 contain Dylight 680/800 Protein Ladder (Pierce). AlexaFluor 790 shows higher non-specific background as evident from the multiple bands visible in lanes 2 and 3.
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