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p53 (TP53) (NM_000546) Human Tagged ORF Clone

Supplier:
Catalogue number:
RC200003
Size:
10 µg
Product is available in:
  • UK
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TP53 (Myc-DDK-tagged)-Human tumor protein p53 (TP53), transcript variant 1

Product Type:

cDNA clones

UNSPSC Code:

41106309

TP53 activity in the over-expression lys

TP53 activity in the over-expression lysate (LY400186) was measured in a colorimetric DNA-binding assay. Double-stranded oligonucleotide containing the p53 consensus DNA-binding sequence was incubated with dilutions of the over-expression lysate and TP53 bound to the oligo was captured onto the surface of a microtiter plate. After washing, bound TP53 was detected with an anti-p53 primary antibody followed by an HRP-labeled secondary antibody. After initial color development, the reaction was quenched and the color intensity was measured at 450nm. Overexpression cell lysates are prepared from HEK293T cells transfected with RC200003 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Western blot validation of overexpressio

Western blot validation of overexpression lysate (Cat# LY400186) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200003 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Coomassie blue staining of purified TP53

Coomassie blue staining of purified TP53 protein (Cat# TP300003). The protein was produced from HEK293T cells transfected with TP53 cDNA clone (Cat# RC200003) using MegaTran 2.0 (Cat# TT210002).

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