Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat. #TA302232). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).
Western blot analysis of ATF4 Antibody (S245) Pab (Cat. #TA302232) pre-incubated without (lane 1) and with (lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.