Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481) (Cat.#TA302216). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481) (Cat#TA302216) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).