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AKT2 Rabbit Polyclonal Antibody

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100 µg
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Rabbit Polyclonal Antibody against AKT2 (S474)

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Western blot analysis of AKT2 (arrow) us

Western blot analysis of AKT2 (arrow) using rabbit polyclonal AKT2 Antibody (S474) (RB11384). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).

Formalin-fixed and paraffin-embedded hum

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 Antibody (S474), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

IF image of C2C12 cell stained with AKT2

IF image of C2C12 cell stained with AKT2 Antibody (S474) (Cat#TA302205).C2C12 cells were incubated with AKT2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).AKT2 immunoreactivity is localized to Cytoplasm significantly.