The phospho-STAT3-Ser727 Pab (Cat. #TA302174) is used in Western blot to detect Ser727-phosphorylated STAT3 in mouse liver tissue lysates collected before (-) or after (+) stimulation obtained by IL-6 injection in mouse portal vein. Data is kindly provided by Drs. E. Bard-Chapeau and G-S. Feng from the Burnham Institute (La Jolla, CA).
IF image of SY5Y cells stained with phospho-STAT3-S727 antibody. SY5Y cells were incubated with TA302174 phospho-STAT3-S727 primary antibody (1:100, 2 h at RT). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue). Note the highly specific localization of the phospho-STAT3-S727 immunosignal to the nucleus and cytoplasm.