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Neurogenin3 (NEUROG3) Mouse Monoclonal Antibody (Clone ID: OTI5A8)

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100 µl
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Anti-Ngn3 (Neurogenin3) mouse monoclonal antibody, clone OTI5A8 (formerly 5A8)

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HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3. Positive lysates LY402827 (100ug) and LC402827 (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500090) at 1:150 dilution at 1:150 dilution.

Immunoprecipitation of NEUROG3 by using

Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.