HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1 (RC211365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1. Positive lysates LY402602 (100ug) and LC402602 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and NOTCH1-Knockout Hela cells (KO, Cat# LC810153) were separated by SDS-PAGE and immunoblotted with anti-NOTCH1 monoclonal antibody TA500078. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).
Figure from citation: Immunohistochemistry for Notch1: Comparative analysis of Notch1 expression between human adenocarcinomas and neuroendocrine tumours (NETs) by using anti-Notch 1 antibody. Nuclear expression is found in the adenocarcinomas (A and B, arrows), but rarely in NETs (A and B, *). Original magnification, (A), ×40; (B), ×200. Dilution: 1:40 View Citation