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NOTCH1 Mouse Monoclonal Antibody [Clone ID: OTI3E12]

Catalogue number:
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TA500078
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£570.00
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Recombinant protein expressed in E.coli corresponding to amino acids 2300-2556 of human NOTCH1

Alternative Names:

AOS5; AOVD1; hN1; TAN1

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Product Description:

Anti-Notch1 mouse monoclonal antibody, clone OTI3E12 (formerly 3E12)

Storage Temperature:

Store at -20°C as received.

Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded thyroid within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded colon within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded pancreas within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded lung within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded kidney within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of kidney using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of lung using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded breast within the normal limits using anti-Notch1 (TA500078) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Notch1 (RC211365, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Notch1. Positive lysates LY402602 (100ug) and LC402602 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and NOTCH1-Knockout Hela cells (KO, Cat# LC810153) were separated by SDS-PAGE and immunoblotted with anti-NOTCH1 monoclonal antibody TA500078. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).
Figure from citation: Immunohistochemist
Figure from citation: Immunohistochemistry for Notch1: Comparative analysis of Notch1 expression between human adenocarcinomas and neuroendocrine tumours (NETs) by using anti-Notch 1 antibody. Nuclear expression is found in the adenocarcinomas (A and B, arrows), but rarely in NETs (A and B, *). Original magnification, (A), ×40; (B), ×200. Dilution: 1:40 View Citation
Anti-Notch1 mouse monoclonal antibody (T
Anti-Notch1 mouse monoclonal antibody (TA500078) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY Notch1 (RC211365)
Immunofluorescent staining of HepG2 cell
Immunofluorescent staining of HepG2 cells using anti-Notch1 mouse monoclonal antibody (TA500078).
Immunofluorescent staining of HT29 cells
Immunofluorescent staining of HT29 cells using anti-Notch1 mouse monoclonal antibody (TA500078).
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