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alpha Actinin (ACTN1) Mouse Monoclonal Antibody [Clone ID: OTI7A4]

Catalogue number:
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TA500072
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
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£533.00
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Applications:

IF, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Human recombinant protein fragment corresponding to amino acids 650-893 of human alpha-actinin (NP_001093) produced in E.coli.

Alternative Names:

BDPLT15

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Product Description:

alpha-actinin (Actinin alpha 1) mouse monoclonal antibody, clone OTI7A4 (formerly 7A4)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACTN1 (RC204924, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACTN1. Positive lysates LY400447 (100ug) and LC400447 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-ACTN1 monoclonal antibody at 1:200 dilution.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ACTN1 monoclonal antibody (1:200).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ACTN1-Knockout HeLa cells (KO, Cat# LC832215) were separated by SDS-PAGE and immunoblotted with anti-ACTN1 monoclonal antibody TA500072 (1:500`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500072)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500072)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500072)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500072)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-ACTN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500072)
Anti-ACTN1 mouse monoclonal antibody (TA
Anti-ACTN1 mouse monoclonal antibody (TA500072) immunofluorescent staining of Hela cells transiently transfected by pCMV6-ENTRY ACTN1 (RC204924).
Immunofluorescent staining of HeLa cells
Immunofluorescent staining of HeLa cells using anti-ACTN1 mouse monoclonal antibody (TA500072).
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