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SSB Mouse Monoclonal Antibody [Clone ID: OTI3H9]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
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Applications:

FC, IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human SSB expression vector

Alternative Names:

La; La/SSB; LARP3

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Product Description:

Anti-SSB mouse monoclonal antibody, clone OTI3H9 (formerly 3H9)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB. Positive lysates LY401091 (100ug) and LC401091 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)
Immunofluorescent staining of HT29 cells
Immunofluorescent staining of HT29 cells using anti-SSB mouse monoclonal antibody (TA500410).
Immunoprecipitation (IP) of SSB by using
Immunoprecipitation (IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Flow cytometric Analysis of Jurkat cells
Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody (TA500410), (Red), compared to a nonspecific negative control antibody, (Blue).
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