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SSB Mouse Monoclonal Antibody (Clone ID: OTI3H9)

Supplier:
Catalogue number:
TA500410
Size:
100 µl
Product is available in:
  • UK
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Anti-SSB mouse monoclonal antibody, clone OTI3H9 (formerly 3H9)

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (RC205013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB. Positive lysates LY401091 (100ug) and LC401091 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SSB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500410)

Immunofluorescent staining of HT29 cells

Immunofluorescent staining of HT29 cells using anti-SSB mouse monoclonal antibody (TA500410).

Immunoprecipitation (IP) of SSB by using

Immunoprecipitation (IP) of SSB by using TrueMab monoclonal anti-SSB antibodies (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Flow cytometric Analysis of Jurkat cells

Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody (TA500410), (Red), compared to a nonspecific negative control antibody, (Blue).