www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI3F12]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£533.00 Shipping is calculated in checkout
Applications:

IF, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Monkey

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human DFFA (NP_004392) produced in E.coli.

Alternative Names:

DFF-45; DFF1; ICAD

image 0
image 1
image 2
Product Description:

DFF45 (ICAD) mouse monoclonal antibody, clone OTI3F12 (formerly 3F12)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates LY418008 (100ug) and LC418008 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody.
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and DFFA-Knockout HeLa cells (KO, Cat# LC833352) were separated by SDS-PAGE and immunoblotted with anti-DFFA monoclonal antibody TA500063 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Anti-DFFA mouse monoclonal antibody (TA5
Anti-DFFA mouse monoclonal antibody (TA500063) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DFFA (RC202879).
Details Cat number & supplier Size Price
Goat anti-Mouse IgG Heavy and Light Chain Cross-Adsorbed Antibody HRP Conjugated A90-216P · Bethyl Laboratories, Inc.
A90-216P
Bethyl Laboratories, Inc.
0.5 mg £124.00
0.5 mg
view