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CD2 Mouse Monoclonal Antibody [Clone ID: OTI4E4]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
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Applications:

FC, IHC, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human CD2 expression vector

Alternative Names:

LFA-2; SRBC; T11

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Product Description:

Anti-CD2 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (RC206612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. Positive lysates LY400671 (100ug) and LC400671 (20ug) can be purchased separately from OriGene.
Flow cytometric analysis of living 293T
Flow cytometric analysis of living 293T cells transfected with CD2 overexpression plasmid (RC206612, Red)/empty vector (PS100001, Blue) using anti-CD2 antibody (TA500404). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500404) (1:500)
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