www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

CD2 Mouse Monoclonal Antibody (Clone ID: OTI4E4)

Supplier:
Catalogue number:
TA500404
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£422.00 Shipping is calculated in checkout

Anti-CD2 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD2 (RC206612, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD2. Positive lysates LY400671 (100ug) and LC400671 (20ug) can be purchased separately from OriGene.

Flow cytometric analysis of living 293T

Flow cytometric analysis of living 293T cells transfected with CD2 overexpression plasmid (RC206612, Red)/empty vector (PS100001, Blue) using anti-CD2 antibody (TA500404). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500404) (1:500)