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MEK4 (MAP2K4) Mouse Monoclonal Antibody (Clone ID: OTI8A8)

Supplier:
Catalogue number:
TA500403
Size:
100 µl
Product is available in:
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Anti-MAP2K4 (MEK4) mouse monoclonal antibody, clone OTI8A8 (formerly 8A8)

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4. Positive lysates LY401058 (100ug) and LC401058 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.

Western blot analysis of extracts (10ug)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-MAP2K4 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)

Anti-MAP2K4 mouse monoclonal antibody (T

Anti-MAP2K4 mouse monoclonal antibody (TA500403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4 (RC206051).

Immunofluorescent staining of HepG2 cell

Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (TA500403).

Immunoprecipitation (IP) of MAP2K4 by us

Immunoprecipitation (IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

HEK293T cells transfected with either RC

HEK293T cells transfected with either RC206051 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K4 antibody (TA500403), and then analyzed by flow cytometry.