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MEK4 (MAP2K4) Mouse Monoclonal Antibody [Clone ID: OTI8A8]

Catalogue number:
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TA500403
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£533.00
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Applications:

FC, IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Mouse; Rat

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

Alternative Names:

JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1

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Product Description:

Anti-MAP2K4 (MEK4) mouse monoclonal antibody, clone OTI8A8 (formerly 8A8)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAP2K4 (RC206051, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP2K4. Positive lysates LY401058 (100ug) and LC401058 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MAP2K4 monoclonal antibody.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-MAP2K4 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MAP2K4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500403)
Anti-MAP2K4 mouse monoclonal antibody (T
Anti-MAP2K4 mouse monoclonal antibody (TA500403) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAP2K4 (RC206051).
Immunofluorescent staining of HepG2 cell
Immunofluorescent staining of HepG2 cells using anti-MAP2K4 mouse monoclonal antibody (TA500403).
Immunoprecipitation (IP) of MAP2K4 by us
Immunoprecipitation (IP) of MAP2K4 by using TrueMab monoclonal anti-MAP2K4 antibodies (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC206051 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAP2K4 antibody (TA500403), and then analyzed by flow cytometry.
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