HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK5 (RC200342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5. Positive lysates LY401536 (100ug) and LC401536 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDK5-Knockout 293T cells (KO, Cat# LC810801) were separated by SDS-PAGE and immunoblotted with anti-CDK5 monoclonal antibody TA500397, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.