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CDK5 Mouse Monoclonal Antibody [Clone ID: OTI2H6]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

FC, IF, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human CDK5 (NP_004926) produced in HEK293T cell.

Alternative Names:

PSSALRE

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Product Description:

CDK5 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDK5 (RC200342, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDK5. Positive lysates LY401536 (100ug) and LC401536 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CDK5 monoclonal antibody.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-CDK5 monoclonal antibody (1:200).
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-CDK5 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CDK5-Knockout 293T cells (KO, Cat# LC810801) were separated by SDS-PAGE and immunoblotted with anti-CDK5 monoclonal antibody TA500397, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Anti-CDK5 mouse monoclonal antibody (TA5
Anti-CDK5 mouse monoclonal antibody (TA500397) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CDK5 (RC200342).
Immunofluorescent staining of HT29 cells
Immunofluorescent staining of HT29 cells using anti-CDK5 mouse monoclonal antibody (TA500397).
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC200342 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDK5 antibody (TA500397), and then analyzed by flow cytometry.
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