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PLK1 Mouse Monoclonal Antibody (Clone ID: OTI1A2)

Supplier:
Catalogue number:
TA500395
Size:
100 µl
Product is available in:
  • UK
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PLK1 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2)

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (RC201795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1. (1:. Positive lysates LY417592 (100ug) and LC417592 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (10ug)

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-PLK1 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PLK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500395)

Immunoprecipitation (IP) of PLK1 by usin

Immunoprecipitation (IP) of PLK1 by using TrueMab monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PLK1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

HEK293T cells transfected with either RC

HEK293T cells transfected with either RC201795 overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody (TA500395), and then analyzed by flow cytometry (1:100).