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Chk2 (CHEK2) Mouse Monoclonal Antibody [Clone ID: OTI10G7]

Supplier:
Catalogue number:
TA500390
Size:
100 µl
Product is available in:
  • UK
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CHEK2 (CHK2) mouse monoclonal antibody, clone OTI10G7 (formerly 10G7)

Product Type:

Antibodies

Species Reactivity:

Human, Monkey, Mouse, Rat, Dog

Antibody Host:

Mouse

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (RC201278, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates LY416128 (100ug) and LC416128 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500390)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CHEK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500390)

Immunofluorescent staining of HT29 cells

Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (TA500390).

Immunoprecipitation (IP) of CHEK2 by usi

Immunoprecipitation (IP) of CHEK2 by using TrueMab monoclonal anti-CHEK2 antibodies (Negative control: IP without adding anti-CHEK2 antibody.). For each experiment, 500ul of DDK tagged CHEK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CHEK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

HEK293T cells transfected with either RC

HEK293T cells transfected with either RC201278 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (TA500390), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells

Flow cytometric Analysis of Jurkat cells, using anti-CHEK2 antibody (TA500390), (Red), compared to a nonspecific negative control antibody, (Blue).