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beta III Tubulin (TUBB3) Mouse Monoclonal Antibody (Clone ID: OTI5H2)

Supplier:
Catalogue number:
TA500047
Size:
100 µl
Product is available in:
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TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)

Product Type:

Antibodies

UNSPSC Code:

12352203

Equivalent amounts of cell lysates (10 u

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TUBB3-Knockout Hela cells (KO, Cat# LC810307) were separated by SDS-PAGE and immunoblotted with anti-TUBB3 monoclonal antibody TA500047. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB3 (RC200755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB3. Positive lysates LY416872 (100ug) and LC416872 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB3 monoclonal antibody at 1:200. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 2 different tissues by using anti-TUBB3 monoclonal antibody (1:500).