Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TUBB3-Knockout Hela cells (KO, Cat# LC810307) were separated by SDS-PAGE and immunoblotted with anti-TUBB3 monoclonal antibody TA500047. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB3 (RC200755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB3. Positive lysates LY416872 (100ug) and LC416872 (20ug) can be purchased separately from OriGene.