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beta III Tubulin (TUBB3) Mouse Monoclonal Antibody [Clone ID: OTI5H2]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
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Applications:

WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Human recombinant protein fragment corresponding to amino acids 1-150 of human TUBB3 (NP_006077) produced in E.coli.

Alternative Names:

beta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; FEOM3; TUBB4

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Product Description:

TUBB3 (beta III Tubulin) mouse monoclonal antibody, clone OTI5H2 (formerly 5H2)

Storage Temperature:

Store at -20°C as received.

Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and TUBB3-Knockout Hela cells (KO, Cat# LC810307) were separated by SDS-PAGE and immunoblotted with anti-TUBB3 monoclonal antibody TA500047. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).
HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBB3 (RC200755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBB3. Positive lysates LY416872 (100ug) and LC416872 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBB3 monoclonal antibody at 1:200. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 2 different tissues by using anti-TUBB3 monoclonal antibody (1:500).
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