HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 (RC222354, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDX1 (1:500).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500038)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500038)
Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY PDX1 (RC222354) using anti-PDX1 antibody (TA500038/green, left). 293T cells transfected with empty vector served as a negative control (right) (1:100).
Immunofluorescent staining of HT29 cells using anti-PDX1 mouse monoclonal antibody (TA500038).
Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PDX1 monoclonal antibody (1:200).