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alpha 1 Antitrypsin (SERPINA1) Mouse Monoclonal Antibody [Clone ID: OTI11G2]

Catalogue number:
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TA500378
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
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£533.00
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Applications:

IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Dog; Monkey

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human SERPINA1 expression vector

Alternative Names:

A1A; A1AT; AAT; alpha1AT; PI; PI1; PRO2275

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Product Description:

Anti-SERPINA1 mouse monoclonal antibody, clone OTI11G2 (formerly 11G2)

Storage Temperature:

Store at -20°C as received.

Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500378)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500378)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500378)
Immunoprecipitation (IP) of SERPINA1 by
Immunoprecipitation (IP) of SERPINA1 by using TrueMab monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SERPINA1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.
HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1. Positive lysates LY400112 (100ug) and LC400112 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-SERPINA1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).
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