www.bioscience.co.uk
Sales & Support: +44 (0)1223 316 855

*

alpha 1 Antitrypsin (SERPINA1) Mouse Monoclonal Antibody (Clone ID: OTI9A1)

Supplier:
Catalogue number:
TA500374
Size:
100 µl
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£422.00 Shipping is calculated in checkout

Anti-SERPINA1 mouse monoclonal antibody, clone OTI9A1 (formerly 9A1)

Product Type:

Antibodies

UNSPSC Code:

12352203

HEK293T cells transfected with either RC

HEK293T cells transfected with either RC202082 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINA1 antibody (TA500374), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500374)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500374)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500374)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500374)

Immunohistochemical staining of paraffin

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500374)

Immunoprecipitation (IP) of SERPINA1 by

Immunoprecipitation (IP) of SERPINA1 by using TrueMab monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SERPINA1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (RC202082, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1. Positive lysates LY400112 (100ug) and LC400112 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (10ug)

Western blot analysis of extracts (10ug) from 7 Human tissue by using anti-SERPINA1 monoclonal antibody ( 1: Uterus; 2: Breast; 3: Brain; 4: Liver; 5: Ovary; 6: Thyroid gland; 7: colon) at 1:200 dilution.