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Cytokeratin 8 (KRT8) Mouse Monoclonal Antibody [Clone ID: OTI1B9]

Supplier:
Catalogue number:
TA500020
Size:
100 µl
Product is available in:
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CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B9 (formerly 1B9)

Product Type:

Antibodies

Species Reactivity:

Human, Monkey, Mouse, Rat, Dog

Antibody Host:

Mouse

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

UNSPSC Code:

12352203

HEK293T cells were transfected with the

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8. Positive lysates LY419425 (100ug) and LC419425 (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

Equivalent amounts of cell lysates (10 u

Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and KRT8-Knockout Hela cells (KO, Cat# LC810123) were separated by SDS-PAGE and immunoblotted with anti-KRT8 monoclonal antibody TA500020, (1:2000. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Anti-KRT8 mouse monoclonal antibody (TA5

Anti-KRT8 mouse monoclonal antibody (TA500020) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8 (RC209570).