Human, Mouse, Rat, Dog
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT18 (RC210049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT18. Positive lysates LY404683 (100ug) and LC404683 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and KRT18-Knockout HeLa cells (KO, Cat# LC831285) were separated by SDS-PAGE and immunoblotted with anti-KRT18 monoclonal antibody TA500016 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.