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L1CAM Mouse Monoclonal Antibody [Clone ID: OTI1H3]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
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Applications:

FC, IF, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.

Alternative Names:

CAML1; CD171; HSAS; HSAS1; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

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Product Description:

L1CAM mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM. Positive lysates LY400150 (100ug) and LC400150 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 3 cell line lysates by using anti-L1CAM monoclonal antibody (1:500).
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and L1CAM-Knockout HeLa cells (KO, Cat# LC832196 ) were separated by SDS-PAGE and immunoblotted with anti-L1CAM monoclonal antibody TA500353 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Anti-L1CAM mouse monoclonal antibody (TA
Anti-L1CAM mouse monoclonal antibody (TA500353) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM (RC211601).
Immunoprecipitation of L1CAM by using Tr
Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC211601 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-L1CAM antibody (TA500353), and then analyzed by flow cytometry.
Flow cytometric analysis of living Hela
Flow cytometric analysis of living Hela cells, using anti-L1CAM antibody (TA500353, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
Flow cytometric analysis of living A375
Flow cytometric analysis of living A375 cells, using anti-L1CAM antibody (TA500353, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).
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