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Neurogenin3 (NEUROG3) Mouse Monoclonal Antibody [Clone ID: OTI3B5]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

IF, IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG2a

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector

Alternative Names:

Atoh5; bHLHa7; Math4B; NGN-3; ngn3

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Product Description:

Anti-NEUROG3 (Neurogenin3) mouse monoclonal antibody, clone OTI3B5 (formerly 3B5)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3. Positive lysates LY402827 (100ug) and LC402827 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500346) at 1:150 dilution.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
Anti-NEUROG3 mouse monoclonal antibody (
Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3 (RC224767).
Immunoprecipitation of NEUROG3 by using
Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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