HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GFAP (RC204548, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GFAP (1:500).
Western blot analysis of extracts (35ug) from 1 cell line lysate by using anti-GFAP monoclonal antibody (1:500).
Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-GFAP mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA500344) (1:500)
Anti-GFAP mouse monoclonal antibody (TA500344) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GFAP (RC204548). (The nuclei were counter-stained in blue.)
Immunofluorescent staining of SNB-19 cells using anti-GFAP antibody (TA500344/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).