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Adenylate Kinase 1 (AK1) Mouse Monoclonal Antibody [Clone ID: OTI8A1]

Catalogue number:
Supplier:
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

FC, IF, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Monkey; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full length human recombinant protein of human AK1 (NP_000467) produced in HEK293T cell.

Alternative Names:

HTL-S-58j

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Product Description:

AK1 (Adenylate Kinase 1) mouse monoclonal antibody, clone OTI8A1 (formerly 8A1)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK1 (RC215130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK1. Positive lysates LY400171 (100ug) and LC400171 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AK1 monoclonal antibody.
Equivalent amounts of cell lysates (10 u
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and AK1-Knockout HeLa cells (KO, Cat# LC810761) were separated by SDS-PAGE and immunoblotted with anti-AK1 monoclonal antibody TA500322 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Anti-AK1 mouse monoclonal antibody (TA50
Anti-AK1 mouse monoclonal antibody (TA500322) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK1 (RC215130).
HEK293T cells transfected with either RC
HEK293T cells transfected with either RC215130 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK1 antibody (TA500322), and then analyzed by flow cytometry.
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