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USP13 Mouse Monoclonal Antibody [Clone ID: OTI4G11]

Catalogue number:
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TA500318
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
  • Ireland
  • Europe
  • USA
  • Rest of World
£533.00
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Applications:

IHC, IP, WB

Antibody Host:

Mouse

Species Reactivity:

Human; Monkey; Dog; Mouse; Rat

Antibody Isotype:

IgG1

Antibody Type:

Monoclonal

Immunogen:

Full-length protein expressed in 293T cell transfected with human USP13 expression vector

Alternative Names:

IsoT-3; ISOT3

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Product Description:

Anti-USP13 mouse monoclonal antibody, clone OTI4G11 (formerly 4G11)

Storage Temperature:

Store at -20°C as received.

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP13 (RC202190, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP13. Positive lysates LY401292 (100ug) and LC401292 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-USP13 monoclonal antibody.
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-USP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunohistochemical staining of paraffin
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-USP13mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500318, Dilution 1:50)
Immunoprecipitation (IP) of USP13 by usi
Immunoprecipitation (IP) of USP13 by using TrueMab monoclonal anti-USP13 antibodies (Negative control: IP without adding anti-USP13 antibody.). For each experiment, 500ul of DDK tagged USP13 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-USP13 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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