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Prominin 2 (PROM2) Mouse Monoclonal Antibody (Clone ID: OTI5E5)

Catalogue number:
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TA500310
Supplier:
OriGene
Size:
100 µl _$$_
Product is available in:
  • UK
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Applications:

WB 1:2000

Antibody Host:

Mouse

Species Reactivity:

Human

Antibody Isotype:

IgG2b

Antibody Type:

Monoclonal

Immunogen:

Protein expressed in 293T cell transfected with human PROM2 expression vector

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Product Description:

Anti-PROM2 (Prominin 2) mouse monoclonal antibody, clone OTI5E5 (formerly 5E5)

HEK293T cells were transfected with the
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PROM2 (RC211605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PROM2. Positive lysates LY403405 (100ug) and LC403405 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug)
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-PROM2 monoclonal antibody (1:200).
Anti-PROM2 mouse monoclonal antibody (TA
Anti-PROM2 mouse monoclonal antibody (TA500310) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PROM2 (RC211605).
Immunoprecipitation (IP) of PROM2 by usi
Immunoprecipitation (IP) of PROM2 by using TrueMab monoclonal anti-PROM2 antibodies (Negative control: IP without adding anti-PROM2 antibody.). For each experiment, 500ul of DDK tagged PROM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PROM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
Details Cat number & supplier Size Price
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A90-216P
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0.5 mg
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